rnascope pretreatment kit advanced cell diagnostics-fluorescent multiplex reagent kit  (Advanced Cell Diagnostics Inc)

Journal: Cell

Article Title: GPC3-Unc5 receptor complex structure and role in cell migration

doi: 10.1016/j.cell.2022.09.025

Figure Lengend Snippet:

Article Snippet: 10 μm Cryo-sections were pre-treated using the RNAscope Universal Pretreatment Kit (Advanced Cell Diagnostics).

Techniques: Recombinant, Staining, RNAscope, Modification, Electron Microscopy, Saline, Protease Inhibitor, Multiplex Assay, cDNA Synthesis, Transfection, Negative Control, SYBR Green Assay, shRNA, Knockdown, Plasmid Preparation, Software, Glycoproteomics

Journal: Stress (Amsterdam, Netherlands)

Article Title: Prefrontal Cortex PACAP Signaling: Organization and Role in Stress Regulation

doi: 10.1080/10253890.2021.1887849

Figure Lengend Snippet: Distribution of PACAP and PAC1 mRNA expression in infralimbic (IL) neurons using RNAscope. A. Cellular labeling of PACAP (green) and PAC1 (magenta, white arrows) is largely distinct, with occasional cells showing colocalization (yellow arrows). B. A large population of projection neurons (VGluT1, red) express PACAP (green, yellow arrows). C. PAC1 (magenta) is expressed in both vGluT1-positive (green, yellow arrow) and vGluT1 negative (white arrow) neurons. A sizable population of vGluT1 neurons are PAC1 negative (blue arrow). D. GABAergic (GAD67) interneurons (red) can express mostly PACAP (green, white arrow) or PAC1 (magenta, blue arrow) alone or coexpress PACAP and PAC1 (yellow arrows). E. While astrocyte processes (GFAP, red) are in proximity to both PACAP (green,yellow arrow) and PAC1 (magenta, white arrows)-containing cells, there is no evidence for colocalization in GFAP-positive cell bodies (blue arrows). F. Preferential expression of PACAP vs.PAC1 mRNA in glutamatergic (vGluT1 positive) neurons in the IL, assessed by particle counts in vGlutT1 mRNA expressing neurons as defined from underlying nuclear stain (DAPI). G. Preferential expression of PAC1 vs. PACAP mRNA in GABAergic IL neurons (GAD67 immunopositive). Note undetectable expression of PACAP in the majority of GAD67 neurons. H. PACAP/PAC1 expression ratio in vGlut1 neurons relative to GAD67 highlights differential expression patterns across cell populations Data as Mean±SEM. ** p<0.005, ***p<0.0001.

Article Snippet: The slides were then permeabilized with the RNAscope Pretreatment Kit (Advanced Cell Diagnostics-Fluorescent Multiplex Reagent Kit-Cat # 320850) to unmask the target RNA.

Techniques: Expressing, RNAscope, Labeling, Staining, Quantitative Proteomics